The TaCslA12 gene expressed in the wheat grain endosperm synthesizes wheat-like mannan when expressed in yeast and Arabidopsis

Mannan is a class of cell wall polysaccharides widespread in the plant kingdom. structure and properties vary according to species organ. The walls cereal grains have been extensively studied due their role processing beneficial effect on human health as dietary fiber. Recently, we showed that mannan wheat (Triticum aestivum) grain endosperm has linear β-1,4-linked mannose residues. aim this work was study biosynthesis function mannan. We deposited early during development, identified candidate biosynthetic genes expressed endosperm. functional unsuccessful therefore our best were heterologous systems. endosperm-specificTaCslA12 gene Pichia pastoris an Arabidopsis thaliana mutant depleted glucomannan led production wheat-like lacking glucose residues with moderate acetylation. Therefore, encodes synthase likely responsible for synthesis